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INDIRECT IMMUNOFLUORESCENCE STUDIES OF SMOOTH MUSCLE ANTIBODIES
Author(s) -
Andersen Paul
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02368.x
Subject(s) - antibody , complement fixation test , conjugate , uterus , staining , biology , chemistry , antigen , titer , immunofluorescence , anatomy , microbiology and biotechnology , endocrinology , immunology , serology , mathematical analysis , genetics , mathematics
The reactions of IgG smooth muscle antibodies were studied in an indirect immunofluorescent (IIF) staining system. In chessboard titrations it was found that a conjugate with a molar F:P ratio of 3.6 gave a plateau titre which was the same as, or higher than that of a conjugate with a molar F:P ratio of 2.2. The plateau endpoint concentration of antibody protein in the conjugates fell within the range from 6.3 to 12.5 mcg/ml. The antibodies were species‐ and organ‐non‐specific, and they could be absorbed with a crude actomyosin extract prepared from human uterus or human kidney. Differences in antigenic strength of different tissues occurred. Rat stomach smooth muscle and rat vessel walls yielded higher plateau titres than rat renal glomeruli. Fixation of the tissue sections gave lower titres than unfixed sections. The antibodies were unable to fix complement in a complement IF system.