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PURINE METABOLISM IN NEISSERIA MENINGITIDIS
Author(s) -
Jyssum Sidsel
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02359.x
Subject(s) - purine nucleoside phosphorylase , adenosine , adenosine deaminase , adenine phosphoribosyltransferase , biochemistry , neisseria meningitidis , adenosine triphosphate , hypoxanthine , purine metabolism , deoxyadenosine , purine , chemistry , adenosine monophosphate , nucleoside , biology , enzyme , bacteria , genetics
Whole cells of Neisseria meningitidis are found to utilize exogenous radioactive adenine with labelling of the adenosine 5′‐monophosphate, adenosine 5′‐diphosphate and adenosine 5′‐triphosphate pools. Radioactive hypoxanthine or adenosine were not found in ethanolic extracts from whole cells. Crude extracts from N. meningitidis were found to have activities corresponding to adenine phosphoribosyltransferase (EC 2.4.2.7). The adenosine 5′‐monophosphate formed was phosphorylated to adenosine 5′‐triphosphate. No activity corresponding to purine‐nucleoside phosphorylase (EC 2.4.2.1) or to adenine deaminase (EC 3.5.4.2) could be demonstrated in crude extracts from N. meningitidis.