Premium
APPLICATION OF INDIRECT IMMUNOFLUORESCENCE, INDIRECT HAEMAGGLUTINATION AND POLYACRYLAMIDE‐GEL ELECTROPHORESIS TO HUMAN T‐MYCOPLASMAS
Author(s) -
Black Finn T.,
KrogsgaardJensen A.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02336.x
Subject(s) - immunofluorescence , indirect immunofluorescence , hemagglutination , mycoplasma , polyacrylamide gel electrophoresis , serotype , antibody , microbiology and biotechnology , biology , haemagglutination inhibition , virology , immunology , biochemistry , serology , enzyme
An indirect immunofluorescence technique using unfixed colonies is applied to human T‐mycoplasmas. The method is found to possess the same degree of specificity as the growth‐inhibition test and to be well suited for the identification and classification of T‐myco‐plasmas. The sensitivity is too low for the detection of antibodies in patient sera. A modification of the indirect haemagglutination test applicable to T‐mycoplasmas was less specific, but owing to its sensitivity it can be recommended as a supplement to the metabolic‐inhibition test in studies of antibodies in patient sera. The polyacrylamide‐gel electrophoresis appeared to be unsuitable for the identification and classification of human T‐mycoplasma serotypes.