Premium
IN VITRO TRANSFORMATION OF MOUSE SPLEEN CELLS BY CASEIN, PHYTOHAEMAGGLUTININ AND ALLOGENEIC CELLS IN CASEIN‐INDUCED AMYLOIDOSIS
Author(s) -
Moesner J.,
Birkeland S. A.,
Ebbesen P.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02325.x
Subject(s) - phytohaemagglutinin , in vitro , amyloidosis , spleen , casein , amyloid (mycology) , transformation (genetics) , immune system , chemistry , biology , microbiology and biotechnology , endocrinology , medicine , immunology , biochemistry , gene , inorganic chemistry
Spleen cells from casein‐treated mice developing amyloid were tested for in vitro reaction to phytohaemagglutinin (PHA), allogeneic lymphocytes (MLC) and casein, using uptake of 14 C‐thymidin as a measure of the transformation. The response to PHA and casein was found to be unaffected by amyloid development, whereas the MLC was depressed in amyloid mice. Our results indicate 1) that amyloid development may be accompanied by elective depression of a certain part of the function of immune cells, 2) that tolerance to the applied casein is not a pre‐requisite for amyloid development.