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AN INDIRECT IMMUNOPEROXIDASE TEST FOR HUMAN ANTIBODIES TO TOXOPLASMA GONDII
Author(s) -
Ourth Donald D.,
Matre Roald,
Helgeland Svein M.,
Tönder Olav
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02306.x
Subject(s) - immunoperoxidase , benzidine , methylene blue , titer , cellophane , virology , hemagglutination , peroxidase , chemistry , antibody , microbiology and biotechnology , enzyme , biology , immunology , biochemistry , catalysis , organic chemistry , photocatalysis , monoclonal antibody
Human serum was applied to formalinized parasites (RH strain) which had been spotted on glass slides. Peroxidase‐labelled rabbit antihuman gammaglobulin and then a diamino‐benzidine‐H 2 O 2 solution were added separately. The brown color of the parasites, resulting from the enzyme‐substrate reaction, was observed with a light microscope. Results of the immunoperoxidase test were compared with those of the methylene blue dye and indirect hemagglutination tests with excellent agreement of titers being obtained with the dye test.