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STREPTOZOTOCIN‐DIABETES IN THE CHINESE HAMSTER
Author(s) -
Wilander Erik
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb00404.x
Subject(s) - islet , streptozotocin , pancreas , nucleolus , chinese hamster , thymidine , necrosis , endocrinology , hamster , pancreatic islets , regeneration (biology) , biology , medicine , cell , diabetes mellitus , chemistry , nucleus , microbiology and biotechnology , dna , biochemistry
One day after injection of 200 mg streptozotocin/kg body weight in Chinese hamsters, necrotic cell debris or marked degenerative changes were seen in the pancreatic islets light microscopically. Both β‐, α 2 ‐ and α 1 ‐cells seemed to be affected. During the first weeks, in particular, signs of regeneration and neoformation of islet tissue were evident in the pancreatic tissue. Labelling with 3 H‐thymidine revealed an increased incorporation of this isotope in islets of diabetic animals. Tubular structures and proliferating buds consisting mostly of “clear cells” were seen. “Clear cells” were also observed sometimes in aggregates in islets with degenerative changes. The high incorporation of labelled thymidine at the same time as “clear cells” were observed in the islet tissue suggest that these cells are involved in islet cell regeneration. After one week, up to about one month, large islets containing hypertrophied cells with a large nucleus and a prominent nucleolus were found. Hydropic degeneration and even necrosis sometimes occurred in these islets. The regenerative activity as well as the hypertrophic islets disappeared at the end of the observation period (3 months) at which time relatively small islets with degranulated β‐cells and irregularly distributed α 2 ‐ and α 1 ‐cells with variable staining properties and nuclear sizes were seen.