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THE USE OF ANTITHYMOCYTIC SERUM IN THE TRANSPLANTATION OF HeLa‐CELLS INTO RATS
Author(s) -
Sorvari Tapani E.,
Arvilommi Heikki
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb00377.x
Subject(s) - hela , immunosuppression , transplantation , body weight , medicine , andrology , subcutaneous injection , cell , intraperitoneal injection , physiology , immunology , endocrinology , biology , biochemistry
The use of rabbit anti‐rat thymocytic serum (ATS) as immunosuppressant was investigated in the heterotransplantation of HeLa‐cells into rats of different ages. The transplantation was carried out by subcutaneous injection of HeLa‐cell suspension. The first intraperitoneal ATS injection was given on the same day and the subsequent injections were given 2, 4, 7, and 10 days later. On day 14, the rats were killed and the tumour growth was estimated by the size of the nodule and several histological criteria. 0.005 ml ATS/g body weight given every time was observed to be sufficient to give a tumour growth in all animals aged less than 2 days. A considerably bigger dose, 0.04 ml ATS/g body weight, did not further enhance the tumour growth or change the host reaction. However, this dose caused a much higher mortality of animals. 0.0005 ml ATS/g body weight did not cause adequate immunosuppression for transplantation of HeLa‐cells. In experiments with older animals given 0.005 ml ATS/g body weight, the transplantation result in 7‐day‐old rats was comparable to that in younger rats, but clearly poorer results were obtained in 24‐day‐old rats. It is concluded that adequate immunosuppression for the growth of HeLa‐cell tumours in rats 7 days old or younger was achieved by a dose of ATS sufficiently small for practical purposes.