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EFFECTS OF SUPPLY AND WITHDRAWAL OF FLUORIDE
Author(s) -
Lemperg Rudolf K.,
Rosenquist Jan B.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb00370.x
Subject(s) - hydroxyproline , fluoride , chemistry , hexosamines , resorption , glycosaminoglycan , endocrinology , ingestion , cortical bone , medicine , femur , bone remodeling , tibia , biochemistry , chromatography , anatomy , glucosamine , surgery , inorganic chemistry
In one series of experiments the effect of fluoride ingestion on the concentration of hydroxyproline and acid glycosaminoglycans in the cortical bone of growing rabbits was studied. During this period, excessive bone formation and resorption occurred. The glycosaminoglycans were quantitatively determined using the cetylpyridiniumchloride precipitation technique in microscale and qualitatively studied by micro‐zone electrophoresis. Concentrations of hexosamines were found to be higher in the femur than in the tibia while the contrary was true for the hydroxyproline contents. Small age‐related changes were observed in the solubility profiles of the chondroitin sulphate. No alterations were found during 14 weeks of fluoride ingestion. In another series, rabbits were given fluoride during 14 weeks until completed growth after which the fluoride supplement was withdrawn and the animals followed for another 24 weeks. During this period, normalization of the bone morphology was established. Neither quantitative nor qualitative changes occurred with respect to bone glycosaminoglycans and hydroxyproline. Chemical analyses and electrophoresis indicated the presence of minor amounts of keratan sulphate in the cortical bone of rabbits.