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SELECTIVE ELIMINATION IN VITRO OF SENSITIVE CELL CLONES IN METHYLCHOLANTHRENE INDUCED SARCOMA BY VINBLASTINE SULPHATE
Author(s) -
Tropé Claes
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb00367.x
Subject(s) - vinblastine , in vitro , incubation , cell , cell culture , transplantation , population , biology , chemistry , microbiology and biotechnology , pharmacology , biochemistry , chemotherapy , medicine , environmental health , genetics
An in vitro short term model was used to study whether vinblastine sulphate could selectively eliminate cells sensitive to it. The effect of the drug was determined as the reduction of the incorporation of H 3 ‐thymidine into drug treated cells compared with control cells. The transplantability of the cells after incubation with and without vinblastine sulphate was examined. At the transplantation of a fresh cell suspension, only a fraction of the injected cells start growing and produce a tumour. When a cell suspension incubated in vitro for four hours is used, still fewer cells survive and produce tumour growth. A significant reduction in drug effect could be demonstrated in tests performed on tumours developed after in vitro incubation with vinblastine sulphate. The most reasonable explanation of this phenomenon is that the vinblastine sulphate treatment selectively eliminates “sensitive” cells and leaves “resistant” cells alive, resulting in a change in composition of the tumour cell population.