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AUTORADIOGRAPHIC STUDIES OF DYSPLASIA AND CARCINOMA IN SITU IN CERVICAL CONES
Author(s) -
Rubio C. A.,
Lagerlöf B.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb00366.x
Subject(s) - carcinoma in situ , dysplasia , in situ , pathology , carcinoma , biology , epithelium , incubation , thymidine , cervical carcinoma , cytoplasm , anatomy , in vitro , chemistry , cervical cancer , medicine , cancer , microbiology and biotechnology , genetics , biochemistry , organic chemistry
Autoradiograms of histological slides of 98 cervical cone specimens with dysplasia and carcinoma in situ were analysed after incubation of tissue samples with 3 H thymidine in vitro. There was a gradual increase in the proportion of labelled cells from normal to carcinoma in situ. Labelling of superficial cell layers occurred in almost all sections from quadrants with carcinoma in situ , in 80 per cent of those with severe dysplasia, in 22 per cent with moderate dysplasia and in 8 per cent with slight dysplasia, while sections from quadrants with normal epithelium were autoradiographically negative in the superficial cell layers. The labelling of superficial cells in slight and moderate dysplasia suggests that, despite cytoplasmic maturation, their nuclei retained the property of less mature and undifferentiated cells, i.e. the capacity of DNA replication. The highest proportion of labelled cells was found in areas with carcinoma in situ with buds.