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DETERMINATION OF ANTISTREPTOLYSIN O BY REVERSED SINGLE RADIAL IMMUNODIFFUSION
Author(s) -
Fasth Anders
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb00240.x
Subject(s) - radial immunodiffusion , hemolysis , agarose , immunodiffusion , chromatography , dilution , chemistry , coefficient of variation , agar gel , microbiology and biotechnology , immunology , antibody , biology , physics , thermodynamics
A diffusion‐in‐gel micromethod for quantitation of ASO is presented. The method is compared with conventional dilution techniques. Non‐reduced ASO and sheep erythrocytes were mixed in an agarose gel. Around holes filled with sera under test zones of inhibited hemolysis appeared when the ASO was reduced. The intra‐ and interplate variation were 5.5 and 26 ASO units. The correlation coefficient between the method presented and that of Cabau & Badin was 0.936.