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DECREASED ONCOGENIC POTENTIAL OF SV40‐TRANSFORMED CELLS GROWN IN DIFFUSION CHAMBERS INTRAPERITONEALLY
Author(s) -
Stillström J.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb00236.x
Subject(s) - in vivo , irradiation , cell culture , embryo , microbiology and biotechnology , biology , in vitro , pi , cancer research , chemistry , genetics , biochemistry , physics , nuclear physics
Small doses (10 4 ) of cells originating from two cloned lines of SV40‐transformed mouse embryo cells, ME1 C9 and ME2 C11, were enclosed in diffusion chambers which were deposited intraperitoneally in syngeneic CBA mice treated in different ways. The cells derived from the chambers after 5–84 days in vivo were less oncogenic than the cells of origin in 26 cases out of 29. The decrease in oncogenic potential was most pronounced (Progression index, PI = 10 ‐1.5 –10 ‐3.7 ) in irradiated animals and least pronounced (PI = 10 ‐0.3 –10 ‐1.0 ) in non‐irradiated tumour‐bearing animals. Cells passaged subcutaneously and harvested from the resulting tumours after 7–27 weeks were more oncogenic than the cells of origin in 20 cases out of 23. A decrease in oncogenic potential was only found in lines derived from tumours induced by a small dose (10 3 –10 4 cells) in irradiated animals (PI = 10 ‐1.4 , ≥10 ‐0.8 ). Lines derived from tumours induced by a large dose (10 7 cells) in irradiated animals or a small dose (10 2 –10 5 cells) in non‐irradiated animals were more oncogenic than the cells of origin (PI = 10 0 –10 4.6 ).