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INDUCTION OF SV40‐TUMOUR IMMUNITY BY SV40‐TRANSFORMED CELLS IN DIFFUSION CHAMBERS
Author(s) -
Stillström J.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb00235.x
Subject(s) - immunity , immunization , spleen , transplantation , immune system , irradiation , chemistry , inoculation , microbiology and biotechnology , immunology , medicine , andrology , biology , physics , nuclear physics
The level of transplantation immunity against syngeneic SV40 tumour cells was studied in CBA mice after implantation of diffusion chambers containing SV40‐transformed cells. The immunization effect was dependent on the duration of chamber deposition, the site of implantation and the pore size of the chambers. 10 5 irradiated ME1 C9‐V15 cells in 0.45 μm chambers deposited for 1 or 3 days subcutaneously induced a significant level of immunity, as shown by challenge 3 weeks later (Sensitivity index, SI = 10 1.6 and 10 1.9 ). After 7 or more than 21 days of deposition the level of immunity at 3 weeks was increased (SI = 10 2.7 and 10 2.5 ) and almost equivalent to that produced by inoculated cells (SI = 10 2.8 and 10 3.0 ). Ten weeks after immunization with cells in diffusion chambers, the immunity had decreased and was weaker than that induced by directly inoculated cells, both when irradiated and non‐irradiated cells were used for immunization. Cells in chambers with 0.1 μm pores were less immunizing than cells in chambers with 0.45 μm pores. Normal spleen cells added to irradiated tumour cells in chambers with 0.1 μm pores caused a significant level of immunity where the tumour cells alone did not. The immunization effect of syngeneic as well as of xenogeneic SV40‐transformed cells was weaker after intraperitoneal than after subcutaneous inoculation and cells enclosed in diffusion chambers deposited intraperitoneally did not immunize at all.