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REGIONAL LYMPH NODE RESPONSE IN RATS TO INJECTION OF SEMI‐ALLOGENEIC LYMPHOCYTES
Author(s) -
Meyer H.,
Heron I.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02267.x
Subject(s) - lymph node , spleen , lymph , lymphatic system , bone marrow , strain (injury) , hindlimb , medicine , immunology , biology , andrology , pathology
Subsequent to injection of F 1 hybrid lymphoid cells into the hind footpads of the parental strain rats the weight of the popliteal lymph nodes increase with a maximum gain 4 to 6 days after the injection. The enlarged lymph nodes obtain weights approximately 4–5 times greater than normal. When lymphocytes in doses between 2 × 10 6 and 20 × 10 6 per footpad are injected, the node weights increase with a slight dose dependency, but weights never reach the magnitude as in local lymph node G.V.H. assays. Cells from different lymphoid organs had varying antigenic effect, blood‐, lymph node‐, and spleen‐lymphocytes were the best stimulators, whereas bone marrow and thymus were less effective. The weights were found to vary depending on the rat strain used as responder. When closely related rat strains were employed as donor‐recipients the weight maximum was found on the same days and was of the same magnitude as in widely disparate strain combinations. F2 hybrid rats responding against major histocompatible injected F1 hybrid lymphocytes did not produce a weight increase at day 5, but the nodes were found enlarged one week later in the postinjection period.