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DNA REPAIR SYNTHESIS IN MOUSE P‐388 CELLS TREATED WITH MITOMYCIN C
Author(s) -
ørstavik Jon
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02265.x
Subject(s) - mitomycin c , bromodeoxyuridine , thymidine , dna synthesis , dna , microbiology and biotechnology , dna repair , biology , chemistry , biochemistry , cell growth , genetics
DNA repair synthesis was studied in mitomycin C treated mouse P‐388 cells grown in suspension culture by measuring the incorporation of 3 H‐thymidine into non‐replicating DNA. Two methods of density labelling with bromodeoxyuridine were used. By the first method, repair synthesis was measured as incorporation of 3 H‐thymidine into light DNA (banding at 1.70 in neutral CsCl) during simultaneous labelling with 3 H‐thymidine and bromodeoxyuridine. Increasing amounts of 3 H‐labelled light DNA were then found after exposure to increasing concentrations of mitomycin C. By the second method, repair synthesis was measured as incorporation of 3 H‐thymidine into hybrid DNA (banding at 1.75 in neutral CsCl) of cells grown in medium containing bromodeoxyuridine prior to treatment with mitomycin C. Exposure to increasing concentrations of the antibiotic then resulted in a progressive accumulation of 3 H‐labelled hybrid DNA. The results indicated that the mouse P‐388 cells carried out repair of DNA lesions after treatment with mitomycin C.