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RAPID IDENTIFICATION OF PROTEUS SPECIES AND PROVIDENCIA BY A SIMPLE TWO‐STEP PROCEDURE
Author(s) -
Closs Otto,
Digranes Asbjörn
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02260.x
Subject(s) - providencia , proteus , identification (biology) , maltose , biology , microbiology and biotechnology , chemistry , biochemistry , enzyme , escherichia coli , botany , gene
One hundred and sixty‐nine strains of Proteus and Providencia were studied. A simplified identification procedure in two steps was applied. In the first step, strains belonging to the Proteus‐Providencia group were identified on the basis of one‐hour tests for phenylalanine deaminase and β‐D‐galactosidase activity. In the second step, differentiation between the four Proteus species and Providencia was based on H 2 S production, urease activity, fermentation of maltose, and ornithine decarboxylase activity (HUMO). Identification based on the HUMO‐scheme accorded well with the final identification based on 19 characters. The HUMO‐scheme is therefore recommended for use in routine diagnostic work.