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THE INFLUENCE OF GROWTH TEMPERATURE ON THE THERMAL DENATURATION OF RIBOSOMES ISOLATED FROM ESCHERICHIA COLI
Author(s) -
Beck Marianne,
Kiel Pauli,
Hansen Erik Wind
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02246.x
Subject(s) - ribosome , hyperchromicity , escherichia coli , denaturation (fissile materials) , chemistry , thermal stability , melting temperature , analytical chemistry (journal) , crystallography , chromatography , biochemistry , materials science , nuclear chemistry , dna , rna , organic chemistry , composite material , gene
The thermal stability of ribosomes isolated from Escherichia coli grown in minimal medium at different temperatures has been determined by measuring the hyperchromicity at 260 nm while gradually increasing the temperature of the ribosomes which were suspended in standard buffer containing 0.01 M Mg++ions. The melting profiles are dependent upon the sample position in the spectrophotometer since the ribosome suspensions become turbid as the temperature is increased. The measurements did not show any difference between the T m values obtained for ribosomes isolated from cells grown at 15° C and 37° C, respectively, but at temperatures above 71° C the denaturation profiles of 70S ribosomes from E.coli grown at 15° C and 37° C are different.