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PURIFICATION AND CHARACTERIZATION OF SEROLOGICALLY ACTIVE CELL WALL SUBSTANCES FROM PLANOCOCCUS STRAINS
Author(s) -
Endresen G.,
Oeding P.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02245.x
Subject(s) - teichoic acid , polysaccharide , cell wall , antiserum , microbiology and biotechnology , biology , agglutination (biology) , agar , bacteria , antigen , chemistry , biochemistry , peptidoglycan , genetics
From the cell walls of four Planococcus strains polysaccharide and murein were separated and purified. The walls contained small amounts of polysaccharides, murein being the major constituent. Teichoic acids were not present. Both chemically and serologically the polysaccharides of two strains seemed to be identical, whereas this polysaccharide and those from the two other strains all were clearly different. Each of the three types of polysaccharides produced a specific precipitation line in agar gel diffusion. Quantitative agglutination with anti‐murein antisera revealed that Planococcus mureins share antigenic determinants with Staphylococcus mureins but also have determinants of their own, which seem to be shared by Planococcus strains.