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PURIFICATION OF VACCINIA HAEMAGGLUTININ
Author(s) -
Gurvin I.,
Haukenes G.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02243.x
Subject(s) - centrifugation , sucrose , antiserum , agar , vaccinia , hela , chromatography , polyacrylamide gel electrophoresis , chemistry , agar gel , sucrose gradient , biology , microbiology and biotechnology , biochemistry , antibody , in vitro , bacteria , immunology , enzyme , recombinant dna , genetics , gene
Vaccinia haemagglutinin was isolated from HeLa cells and purified by a two step ultra‐centrifugation procedure. In the first step the crude haemagglutinin was adjusted to 10 per cent sucrose and layered on top of 40 per cent sucrose. Fractions containing haemagglutinin were adjusted to 50 per cent sucrose, and 40 per cent sucrose was layered on top of this, The haemagglutinin which was recovered in the top fractions, contained no other proteins detectable by polyacrylamide electrophoresis and gave no precipitation line on double diffusion in agar against a vaccinia antiserum. The density range in sucrose was 1.08 to 1.18 g/cm 3 , indicating the lipoprotein nature of the material.