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PHAGOCYTOSIS OF SALMONELLA TYPHIMURIUM 395 MR10 BY RABBIT POLYMORPHONUCLEAR LEUCOCYTES: FORMATION OF PHAGOLYSOSOMES DEMONSTRATED BY ZONAL SEPARATION
Author(s) -
Tagesson Christer,
Stendahl Olle
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02233.x
Subject(s) - phagocytosis , bacteria , salmonella , centrifugation , phagosome , enzyme , microbiology and biotechnology , biology , chemistry , biochemistry , genetics
After allowing phagocytic uptake of bacteria, rabbit polymorphonuclear leucocytes were disintegrated and fractionated by zonal centrifugation. Two different subcellular particles were obtained containing high relative activities of lysosomal marker enzymes. One particle displayed the sedimenting characteristics of lysosomes, the other sedimented with radio‐actively labelled bacteria subjected to phagocytosis. The properties of the latter particles and the conditions concerning their formation were those expected of phagolysosomes. The phagolysosomes showed a peak at a density = 1.19, whereas both lysosomes and nonphagocytosed bacteria were recovered at a density = 1.25. The results are discussed in relation to the analysis of host‐parasite interaction at a subcellular level.