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DISINTEGRATION OF SINGLE CELLS IN SUSPENSION
Author(s) -
Tagesson C.,
Stendahl O.,
Magnusson K.E.,
Edebo L.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02231.x
Subject(s) - centrifugation , granule (geology) , differential centrifugation , chemistry , suspension (topology) , extrusion , biophysics , microbiology and biotechnology , chromatography , biology , materials science , biochemistry , composite material , mathematics , homotopy , pure mathematics
Disintegration of rabbit polymorphonuclear leucocytes in suspension is achieved by extrusion under controlled pressure. The operating pressure is the key factor influencing the degree of disintegration. The number of cells disintegrated increases with higher pressure. At 35 kp/cm 2 , 80–90 per cent of the cells are disintegrated. The disintegrated material was subsequently analysed by following the subcellular distribution of leucocyte A and B granule marker enzymes. The analysis reveals that although 80–90 per cent of the cells are disintegrated, a high degree of subcellular integrity remains. Leucocyte A and B granules, abundantly present in the disintegrated material, were separated from soluble components by zonal centrifugation. Extrusion under controlled pressure as a cell disintegration method is discussed, particularly with reference to the study of polymorphonuclear leucocyte structure and function.