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EVALUATION OF TWO DIFFERENT ANTIGEN PREPARATIONS IN THE FLUORESCENT ANTIBODY TEST FOR ANTINUCLEAR ANTIBODIES (ANA)
Author(s) -
Bergquist N. R.,
Danielsson D.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02229.x
Subject(s) - anti nuclear antibody , thyroid , staining , antibody , immunofluorescence , pathology , antigen , medicine , blood smear , indirect immunofluorescence , direct fluorescent antibody , immunology , autoantibody , malaria
Two different antigen preparations of human origin (sectioned thyroid cells and blood smears) were compared in the indirect immunofluorescence (IF) test for antinuclear antibodies (ANA). Two sets of sera were investigated with identical reagents at two laboratories equipped with similar optical systems. The reproducibility was 94 per cent if thyroid sections were used at both places but dropped to 72 per cent if one laboratory substituted thyroid sections for blood smears. All sera showing speckled staining with thyroid sections were negative with blood smears. Tests carried out on blood smears resulted consistently in higher ANA titres as compared with results obtained with thyroid sections. Low ANA titres were found in 5 per cent of healthy blood donors if blood smears were used but only in 1 per cent with sectioned thyroid cells. Tissue sections are recommended for routine screening of ANA.