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HAEMOLYTIC ACTIVITY OF VARIOUS STRAINS OF ACINETOBACTER
Author(s) -
Lehmann Vidar
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02226.x
Subject(s) - acinetobacter calcoaceticus , haemolysis , microbiology and biotechnology , hemolysin , extracellular , biology , lysis , hemolysis , lecithinase , intracellular , acinetobacter , biochemistry , enzyme , virulence , antibiotics , immunology , gene
Extracellular and intracellular haemolysin (phospholipase C) of Acinetobacter calcoaceticus have been examined during bacterial growth in a chemically defined medium. The haemolytic activities of various strains of Acinetobacter , grown in broth media, have been compared. Intracellular haemolytic activity was detected only at the early stage of growth. Release of haemolysin from the cells started at this point and reached its maximum at the end of the logarithmic phase of growth. In A. calcoaceticus (anitratus), 6 out of 17 strains were haemolytic to human red cells, and in A. lwoffi 2 out of 13 strains. In broth culture filtrates, two distinct types of haemolysis occurred. In some strains, haemolysis increased after cooling (hot‐cold effect), while in others a regular, direct haemolysis occurred at 37° C, with no detectable increase in lysis after cooling. Both types of activity were inactivated by heating and by ethylendiaminetetraacetate, and activated by Mg2+ and albumin. All haemolytic filtrates examined released acid soluble phosphorus from lecithin. The two types of haemolytic activities showed immunological differences.