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RABBIT ANTI RAT LYMPHOCYTE SERUM: IN VITRO ACTIVITY TOWARDS MACROPHAGES IN MONOLAYER
Author(s) -
Jakobsen Arnt
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02214.x
Subject(s) - in vitro , cytotoxic t cell , antibody , chemistry , complement (music) , macrophage , immunology , lymphocyte , microbiology and biotechnology , biology , biochemistry , complementation , gene , phenotype
A method for demonstration of cytotoxic antibodies in antilymphocyte serum (ALS) towards macrophages is described. Peritoneal macrophages were cultured in a medium containing 3 H‐Leucine for 16 hours. Substitution of the culture medium with a medium containing ALS and active complement, resulted in rapid release of radioactivity into the medium, compared to the slow spontaneous release in control experiments. The release of label was dependent on active complement.