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STUDIES ON A FACTOR IN INTESTINAL STRANGULATION FLUID FROM GERMFREE AND CONVENTIONAL RATS INHIBITING SERUM BACTERICIDAL ACTIVITY ON ESCHERICHIA COLI
Author(s) -
Trippestad Arne,
Midtvedt Tore
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02213.x
Subject(s) - escherichia coli , sephadex , microbiology and biotechnology , chemistry , exudate , opsonin , small intestine , chromatography , biology , biochemistry , phagocytosis , enzyme , botany , gene
Exudate collected from a strangulated loop of intestine in conventional and germfree rats has the property of inhibiting the opsonic and bactericidal activity of normal rat serum on Escherichia coli. Using release of radioactivity from 32 P‐labelled E. coli as an indicator of bactericidal activity, the inhibitory property of strangulation fluid on serum bactericidal activity has been subjected to further studies. The inhibitory factor (FIB) resisted heat treatment for 30 min up to 100°C, and did not disappear following dialysis. Chromatography of strangulation fluid from germfree rats on Sephadex G‐100 indicated that FIB had a molecular weight higher than 100 000. Homogenates from intestinal mucosal cells contained FIB, while intestinal contents contained FIB of considerably stronger activity. The results indicated that high molecular substances of intestinal origin were responible for the FIB activity of rat strangulation fluid.