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IMMUNODIFFUSION STUDIES OF PNEUMOCOCCAL ANTIGENS WITH SPECIAL REFERENCE TO SEROTYPE 27
Author(s) -
Broholm K.A.,
Holmgren J.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02210.x
Subject(s) - polysaccharide , serotype , strain (injury) , immunodiffusion , microbiology and biotechnology , antigen , precipitin , chemistry , ouchterlony double immunodiffusion , electrophoresis , biology , chromatography , biochemistry , antiserum , immunology , anatomy
The antigenic pattern of a pneumococcal strain of serotype 27 used as model was studied by immunodiffusion methods and found to show a complex antigenic structure with a least 21 separate precipitinogens. Strains belonging to type 11, 28 and 29 contained most of the precipitinogens present in the model strain while the antigenic composition of type 3 strain differed more from this strain. Double diffusion studies of capsular polysaccharides prepared by conventional methods revealed the presence of contaminating C‐polysaccharide. Within the tested pH range 4–9 the capsular polysaccharides migrated toward the anode. The electrophoretic migration of the C‐polysaccharide varied with pH. At pH 4–5 it moved slowly toward the cathode which made it possible, by preparative electrophoresis, to obtain capsular polysaccharide free from the contaminating C‐polysaccharide. This procedure is suggested as a final step in isolation of capsular polysaccharides for serological work.