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AGGLUTINATION AND IMMUNOFLUORESCENCE STUDIES ON SURFACE ANTIGENS OF S. AUREUS
Author(s) -
Forsum Urban
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02209.x
Subject(s) - staphylococcus aureus , antigen , immunofluorescence , agglutination (biology) , serology , microbiology and biotechnology , antibody , typing , trypsin , biology , protein a , chemistry , virology , bacteria , immunology , biochemistry , enzyme , genetics
Serological typing of 200 S. aureus strains from clinical specimens has been performed with the Oeding‐Haukenes factor sera. Since weak agglutinations were recorded, serologically reacting antigens were studied in immunofluorescence with FITC‐labelled type‐specific anti‐ S. aureus F(ab') 2 ‐fragments of IgG and IgM and TRITC‐labelled normal IgG to detect protein A by its reaction with the Fc‐part of IgG. Protein A was found to be unevenly distributed among the S. aureus cells derived from a single colony and, by trypsin digestion of the bacteria, type‐specific antigens were found to be masked by the common cell surface constituent, protein A.