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ANALYSIS OF THE ELECTROPHORETIC PATTERN OF MYCOPLASMA PROTEINS FOR THE IDENTIFICATION OF CANINE MYCOPLASMA STRAINS
Author(s) -
Rosendal S.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02204.x
Subject(s) - electrophoresis , incubation , mycoplasma , biology , microbiology and biotechnology , biochemistry
The influence of medium composition and cultivation time on the electrophoretic pattern of mycoplasma proteins has been investigated. It was found that media containing horse serum are unsuitable because increasing amounts of medium protein are harvested together with the mycoplasmas during incubation. The medium protein gives rise to unspecific electrophoretic bands which grow more numerous and intense during incubation. These disadvantages were not found in a medium containing PPLO serum fraction. An examination of the electrophoretic patterns of 25 M. canis , 16 M. edwardii and 12 M. spumans strains demonstrated that interspecies differences were more pronounced than intraspecies variations. It is concluded therefore that the electrophoresis method may be used as an aid in the classification of mycoplasmas isolated from dogs.