Premium
SEROLOGICAL CROSS‐REACTIONS BETWEEN DIFFERENT BRUCELLA SPECIES AND YERSINIA ENTEROCOLITICA
Author(s) -
Hurvell B.,
Lindberg A. A.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02194.x
Subject(s) - yersinia enterocolitica , chemistry , monosaccharide , periodate , reactivity (psychology) , cross reactivity , heptose , galactose , immunodiffusion , hapten , microbiology and biotechnology , mannose , phenol , antigen , biochemistry , organic chemistry , cross reactions , bacteria , biology , genetics , medicine , alternative medicine , pathology , mutant , gene
The cross‐reactivity of lipopolysaccharides (LPS) in Brucella abortus and Yersinia enterocolitica type IX recovered from the aqueous and phenol phases of the hot phenol‐water method was examined in immunodiffusion tests. The antigens and the cross‐reactivity were unaffected by heating and by treatment with lipolytic and proteolytic enzymes, but periodate oxidation was found to destroy the precipitating ability. This indicates the carbohydrate nature of the cross‐reacting antigenic determinants. The monosaccharide composition of the fractions was estimated using gas liquid chromatography of peracetylated alditol acetates. The LPS of B. abortus and Y. enterocolitica type IX had glucose and galactose as common monosaccharide constituents. In addition, the LPS of B. abortus contained mannose and that of Y. enterocolitica type IX heptose. The possibility of having the cross‐reactivity localized in O‐specific side‐chains is discussed.