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SEROLOGICAL CROSS‐REACTIONS BETWEEN DIFFERENT BRUCELLA SPECIES AND YERSINIA ENTEROCOLITICA
Author(s) -
Hurvell B.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb02193.x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , chemistry , brucella , serology , lipopolysaccharide , biology , antibody , bacteria , immunology , brucellosis , genetics
Hot phenol‐water extraction of Brucella abortus (B.a.) and Yersinia enterocolitica type IX (Y.e.) cells was performed. The results from the chemical analyses of fractions 3 (F3) and 5 (F5), which were recovered from the aqueous and the phenol phase, respectively, show that these fractions contain lipopolysaccharide complexes. A clear‐cut difference was noted in the yield expressed as the obtained dry weights from fractions F3 and F5 for B.a. and Y.e. For B.a. the yield was greater in F5 than in F3. The relation was about 3:1. For Y.e. the reverse was the case, the relation being about 1:14. The F5 fractions from both B.a. and Y.e. were highly immunogenic. They induced leucopenia and leucocytosis if injected into rabbits. This indicates that both fractions have an endotoxin effect. Both fractions contained O‐somatic agglutinogen, which, if injected into rabbits, gave rise to both primary and secondary immune responses. The antibodies produced agglutinated both B.a. and Y.e. bacteria. In contrast, neither the F3 fraction from B.a. nor that from Y.e. induced leucocytosis or gave rise to agglutinins in rabbits.