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AN IN VITRO STUDY OF THE EFFECT OF CYTOSTATIC DRUGS ON DNA SYNTHESIS IN METHYLCHOLANTHRENE INDUCED MOUSE SARCOMAS AND IN RAT WALKER 256 TUMOURS
Author(s) -
Håkansson Leif,
Tropé Glaes
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1973.tb00501.x
Subject(s) - melphalan , methylcholanthrene , in vitro , cytosine , dna , vinblastine , carcinogen , nitrosourea , biology , microbiology and biotechnology , cancer research , chemistry , pharmacology , chemotherapy , biochemistry , genetics
In the present paper, an in vitro test model was used to study the effect of three cytostatic drugs on the DNA synthesis of experimental tumours: methylcholanthrene induced primary mouse sarcomas, transplanted methylcholanthrene induced sarcomas (tenth generation), and Walker 256 rat adenocarcinoma. A suspension of single cells and small tissue fragments was prepared and incubated with the following drugs: melphalan, vinblastine sulphate, and cytosine arabinoside. The effects of the three drugs were measured as the differences in incorporation of tritiated thymidine in drug‐containing tubes and control tubes. The effect of the drugs on the methylcholanthrene induced primary sarcomas showed a great variation which reasonably is not due to technical error as it is markedly reduced if serially transplanted sarcomas or rat Walker tumours were studied. A correlation of the effects between melphalan and cytosine arabinoside was observed for the sarcomas.