Premium
THE IN VITRO MATURATION OF THE EMBRYONIC CHICKEN THYMUS:
Author(s) -
SäLLSTRöM Jan F.,
Aim Gunnar V.
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1973.tb00479.x
Subject(s) - phytohaemagglutinin , concanavalin a , biology , in vitro , organ culture , embryo , embryonic stem cell , lymphocyte , andrology , phytohemagglutinins , immunology , microbiology and biotechnology , lymphocyte activation , immune system , biochemistry , medicine , t cell , gene
Thymic anlagen of 10‐day‐old chick embryos were allowed to mature for 10 days in organ culture. Lymphocyte suspensions were prepared from such anlagen with a mean yield of 2.85 ° 10° cells per anlage. If subcultivated, such lymphocytes were stimulated by the lymphocyte mitogens Phytohaemagglutinin (PHA) and Concanavalin A (Con A). These results suggest in particular that lymphocytes may acquire their reactivity to PHA and Con A in the thymic micro‐environment. In general the usefulness of the organ culture technique for the clarification of thymic functions is indicated.