Premium
ARTEFACTUAL STAINING OF THE PERIPHERAL ZONE OF NEEDLE BIOPSIES
Author(s) -
Lyon Hans,
PRENTø Poul
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1973.tb00471.x
Subject(s) - staining , stain , negative stain , cytoplasm , eosin , pathology , feulgen stain , chemistry , nucleoprotein , h&e stain , microbiology and biotechnology , cell , biophysics , biology , biochemistry , electron microscope , dna , medicine , physics , optics
A description of artefactual staining in 156 needle biopsies of the liver is given. The following representative methods were employed: Alcian blue'basic fuchsin mixture pH 3, fast green FCF pH 8, azure A'eosin Y mixture pH 5, gallocyanin'chromalum, Feulgen procedure and aluminium'haematein. Changes in stainability were seen in the 3–15 most peripheral cell layers and affected both cell nuclei and cytoplasm as well as sinusoidal lining. A dissociation of nucleoprotein and denaturation of protein in the outermost layers of the biopsy effected by the needle is suggested as being the cause of the altered stain‐ability. The consequences of these findings for the evaluation of needle biopsies is discussed.