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ANALYSIS OF THE VARIATION IN LYMPHOCYTE RESPONSE TO PHA IN NORMAL SUBJECTS
Author(s) -
Hagen Claus,
Frøl Anders
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb00219.x
Subject(s) - thymidine , liquid scintillation counting , transformation (genetics) , lymphocyte , dna synthesis , dna , biology , medicine , microbiology and biotechnology , endocrinology , andrology , chemistry , immunology , genetics , radiochemistry , gene
Among normal persons, a marked variation in DNA synthesis is observed in lymphocyte culture stimulated with PHA. We have studied some of the factors that may be responsible for this normal variation. Liquid scintillation counting of the uptake of tritiated thymidine in cultured cells stimulated by PHA was taken as a measure of the DNA synthesis. 1. A study of 53 normal men and 42 normal women showed that the transformation was correlated to age, but not to sex. 2. Six normal subjects were followed from four to twelve months. Although the lymphocyte transformation varied with time, the level of transformation remained constant. 3. A study of the transformation in seven pairs of monozygous twins and in five pairs of dizygous twins failed to show a genetic influence. 4. Finally, autoradiography and liquid scintillation counting were carried out on the same cultures. It appeared that the transformation as measured by liquid scintillation is propartional to the number of cells synthesizing DNA.