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THE EFFECT OF TRYPSIN ON CELL SUSPENSIONS AND CULTURE SUPERNATANTS OF STRAINS OF CLOSTRIDIUM BOTULINUM TYPES B AND E
Author(s) -
Tjaberg Tore Bjarne
Publication year - 1973
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1973.tb00209.x
Subject(s) - clostridium botulinum , trypsinization , trypsin , microbiology and biotechnology , toxin , clostridium , clostridiaceae , chemistry , cell culture , biology , biochemistry , bacteria , enzyme , genetics
The influence of trypsin on cell suspensions and culture supernatants of non‐proteolytic strains of Clostridium botulinum types B and E has been investigated. Tryptic activation of cell suspensions of the Clostridium botulinum type B strains increased the amount of toxin considerably compared to the amount of toxin produced after trypsinization of the supernatants. This was not the case with Clostridium botulinum type E. The length of the tryptic activation period influenced the toxicity of cell suspensions and supernatants.