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QUANTITATIVE STUDIES OF THE RENAL CORPUSCLES II: A METHODOLOGICAL STUDY
Author(s) -
Sorensen F. Hanberg,
Ledet T.
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1972.tb00342.x
Subject(s) - reproducibility , stereology , mathematics , pathology , statistics , medicine
The camera lucida drawing method with planimetric area measurements was compared with direct microscopy and systematic point count in a quantitative study of glomeruli, the study including differential counts of nuclei and determination of the total and mesangial areas. Good reproducibility was found using both methods, but the camera lucida drawing method was found to be at least three times more time‐consuming than direct microscopy for total and differential counts of nuclei and systematic point count for mesangial area determination. Measurement of total glomerular area was done planimetrically, by point counting and by determination of the area as an ellipse or a circle. The results obtained planimetrically and by point counting did not differ significantly, whereas the difference between the results of the planimeter measurements and the results obtained by applying the formula of an ellipse or a circle on glomerular sections was found to be statistically significant. If central sections were compared with random sections of glomeruli, deviation of results was found to be greater in the random sections, and it was calculated that 10 central and about 30 random sections have approximately the same value in quantitative examination of a renal tissue specimen. Thus, it appears advisable to use direct microscopy for total and differential counts of nuclei, systematic point count for mesangial area determination, planimetric measurement or point counting for total glomerular area determination and to apply this method on central sections of glomeruli.