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THE PROLIFERATION KINETICS OF L 1210 ASCITES TUMOUR
Author(s) -
Dombernowsky Per
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1972.tb00322.x
Subject(s) - doubling time , thymidine , life span , mitosis , dna synthesis , cell growth , ascites , biology , andrology , microbiology and biotechnology , labelling , cell cycle , kinetics , cell division , generation time , immunology , cell , dna , medicine , biochemistry , population , physics , environmental health , quantum mechanics , evolutionary biology
The proliferation kinetics of leukemia L 1210 ascites tumour grown in DBA/2 × NMRI mice was determined. Using the host life‐span method we found a tumour doubling time of 11.7 hours. The doubling time determined by cell counting was 10 hours from day 4–6, 25 hours between day 6 and 7 and about 75 hours from day 7–9 with a standard inoculum of 10 5 cells. Autoradiography of tumour cells after Thymidine 3 H labelling on day 6 after transplantation showed a cell cycle time of 22 hours derived from the per cent labelled mitoses curve. The time spent in DNA synthesis was 15 hours. Continuous labelling showed a growth fraction very near 1. The discrepancy between our data and the data published by other authors is discussed. Neither host life‐span of the mice nor cell doubling time were measurable affected with a dose of 1.0 μCi Thymidine 3 H per gram of mouse used in the studies.