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A SENSITIVE IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING BLOOD GROUP SUBSTANCES A AND B
Author(s) -
Dabelsteen E.,
Rygaard J.
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1972.tb00302.x
Subject(s) - antiserum , immunofluorescence , staining , agglutination (biology) , group a , antibody , epithelium , conjugate , microbiology and biotechnology , indirect immunofluorescence , chemistry , pathology , biology , medicine , immunology , mathematical analysis , mathematics
In order to select a sensitive method for demonstrating blood group substances A and B in sections of oral epithelium, biopsies and exfoliated cells from the oral epithelium were investigated with both a double‐layer immunofluorescence (IF) staining method and the mixed cell agglutination (MCA) reaction. Eighteen anti‐A and 16 anti‐B sera were tested. Fourteen anti‐A sera were able to react in the IF method and only 9 in the MCA reaction. All tested anti‐B sera produced positive IF staining; eleven also reacted in the MCA reaction. Blood group antisera could in most cases be used in higher titres in the IF method than in the MCA technique. In the IF method, porcine antihuman IgG/FITC and porcine antihuman IgM/FITC were used as the second layers. Blood group antisera could generally, but not invariably, be used in higher titres with antihuman IgM/FITC. The importance of careful matching of blood group antiserum and conjugate is emphasized.