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QUANTITATIVE STUDIES OF THE RENAL CORPUSCLES I: INTRAGLOMERULAR, INTERGLOMERULAR AND INTERFOCAL VARIATION IN THE NORMAL KIDNEY
Author(s) -
Sørensen F. Hanberg
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1972.tb00276.x
Subject(s) - glomerulus , kidney , kidney glomerulus , pathology , anatomy , biology , glomerulonephritis , medicine , endocrinology
A quantitative method based on camera lucida drawing and planimetry was used to evaluate intraglomerular, interglomerular and interfocal variation with regard to the following parameters: 1) differential number of glomerular nuclei, 2) mesangial area in percentage of total area, 3) total number of nuclei per 1.000 μ 2 of total area and 4) number of mesangial nuclei per 1.000 μ 2 of mesangial area. Study of intraglomerular variation revealed that, as far as the above‐mentioned parameters are concerned, a central part is found to comprise approximately half of the glomerulus where values are relatively constant. Histograms based on results obtained in 141 normal glomeruli from the same kidney showed a reasonably good approximation to a normal distribution for all parameters concerned. Interfocally, a statistically significant difference in nuclear density could be demonstrated, but this was not the case as regards differential counts and percentage of mesangial area. Comparison of a tissue specimen with subcapsular glomeruli with a tissue specimen with juxtamedullar glomeruli demonstrated no difference with regard to the parameters studied, but the largest glomeruli were found at juxtamedullary sites. It does not appear to matter whether tissue specimens for use in a normal material are removed as small wedges of tissue or as aspiration biopsies. Compressed glomeruli near the edge of the specimens should be excluded.