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STUDIES ON THE LYMPHOCYTES IN THE INTESTINAL EPITHELIUM OF THE CHICKEN
Author(s) -
Bäck Ove
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1972.tb00273.x
Subject(s) - labelling , lamina propria , epithelium , thymidine , mitotic index , intestinal epithelium , biology , basal (medicine) , pathology , mitosis , endocrinology , biochemistry , in vitro , microbiology and biotechnology , medicine , genetics , insulin
Seven‐week‐old male chickens were injected intravenously with 3 H‐thymidine. The presence of labelled lymphocytes in the ileal epithelium was studied by means of autoradiography at various times after injection. There was a significant increase in the labelling index, i.e. the percentage of labelled epithelial lymphocytes, here called theliolymphocytes, between 1 hour and 48 hours after labelling. This increase was considered to be due to both mitosis of labelled theliolymphocytes and immigration of labelled lymphocytes from the lamina propria. During the following 4 days the labelling index decreased. The labelling index in top and base regions of the villi did not differ. The labelling indices of the theliolymphocytes 48 hours and 6 days after 3 H‐TdR administration were significantly higher than the percentages of the labelled peripheral blood lymphocytes at corresponding times. This finding suggests an accumulation of labelled lymphocytes in the intestinal epithelium. In the top regions of the villi, where most of the absorption from the intestine takes place, there was a significantly higher proportion of labelled theliolymphocytes in the apical zone than in the intermediate and basal zones of the epithelium. No such difference was found in the base regions of the villi. The significance of these findings are discussed in relation to current views on the association of epithelial cells and lymphocytes during the development of lymphoid tissues.