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Studies of a Li‐Hapten Isolated from Cell‐Walls of the Rough Mutant Salmonella Typhimurium 395 MR10
Author(s) -
Lindberg A. A.,
Holme T.,
Hellerqvist C. G.,
Svensson S.
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00202.x
Subject(s) - hapten , cytoplasm , cell wall , salmonella , mutant , sephadex , polysaccharide , chromatography , chemistry , ribonuclease , fraction (chemistry) , lysozyme , strain (injury) , sepharose , biochemistry , biology , antigen , bacteria , enzyme , rna , genetics , gene , anatomy
An O‐haptenic material (LI‐fraction) was isolated from cytoplasmic and cell‐wall preparations of the rough mutant Salmonella typhimurium 395 MR10. The yield from the cell‐wall preparation, virtually free from cytoplasmic contamination, was approximately ten times higher than that from the cytoplasmic preparation. The LI‐fraction obtained from cell‐walls was less contaminated with extraneous polysaccharide material. After purification of the LI‐fraction with ribonuclease treatment and Sephadex G‐100 gel chromatography structural studies using methylation analysis could be performed. The hapten showed the same structural features as the O side‐chains of the parent strain S. typhimurium 395 MS. The average number of repeating units in the hapten was 23 as compared to 11 in the O side‐chain of the parent strain. Molecular weight determination with Sepharose 6 B gel chromatography revealed that the LI‐fraction was polydisperse with a weight average of 26,300 and a number average of 21,800.