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LABELLING OF DNA AND RNA FROM THYMINE AND THYMIDINE IN NEISSERIA MENINGITIDIS
Author(s) -
Jyssum Sidsel
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00166.x
Subject(s) - thymine , thymidine , labelling , dna , kinetics , rna , neisseria meningitidis , chemistry , strain (injury) , biochemistry , stereochemistry , biology , genetics , bacteria , gene , physics , anatomy , quantum mechanics
Radioactivity was taken up from labelled thymine and thymidine by whole cells of Neisseria meningitidis strain M1, and both DNA and RNA were labelled. The labelling from substrates carrying the radioactivity in the C‐2 position and in the methyl group was compared. The uptake of radioactivity from thymine uniformly labelled, and from thymidine with label in the 6‐position was also measured. The incorporation of radioactivity varies with the location of the label in the molecule, and the uptake kinetics for the compounds with the label in the C‐2 position is different from the uptake kinetics of the compounds with label in other positions. The findings point to a utilization of break‐down products from thymine and thymidine.