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THE EFFECT OF METABOLIC INHIBITORS ON THE PRODUCTION OF VACCINIA HAEMAGGLUTININ
Author(s) -
Aasen Jorulf,
Haukenes Gunnar
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00155.x
Subject(s) - vaccinia , virology , poxviridae , biology , orthopoxvirus , modified vaccinia ankara , virus , protein biosynthesis , dna synthesis , bromodeoxyuridine , chemistry , dna , microbiology and biotechnology , biochemistry , gene , recombinant dna , cell growth
When synthesis of vaccinia DNA was inhibited, no haemagglutinin (HA) was produced. The antiviral compound isatin‐β‐thiosemicarbazone (IBT) also arrested the formation of vaccinia HA. It is therefore concluded that the synthesis of vaccinia HA depends on late protein synthesis. We have confirmed earlier findings that 5‐bromodeoxyuridine (BUdR) does not inhibit HA production in doses which completely arrest production of infectious virus. In contrast to earlier findings, mitomycin C inhibited HA production in our experiments. The new antiviral compound rifampicin had no or little effect upon vaccinia HA production.