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ANTIGEN‐FREE MEDIUM FOR CULTIVATION OF HAEMOPHILUS INFLUENZAE , AFH‐MEDIUM
Author(s) -
BraneforsHelander Paula
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00151.x
Subject(s) - yeast extract , chemistry , sephadex , haemophilus influenzae , chromatography , growth medium , yield (engineering) , microbiology and biotechnology , liquid medium , chemically defined medium , antigen , food science , bacteria , biochemistry , in vitro , biology , fermentation , materials science , enzyme , immunology , metallurgy , genetics , antibiotics
An antigen‐free medium, AFH medium, for cultivation of H. influenzae is described. The medium is prepared from trypticase‐yeast autolysate, filtrated through a Sephadex G‐25 column. For the medium base only the fractions containing low molecular material were utilized. Addition of different amounts of salt and buffer was tested and the effect of the initial pH of the medium was studied. The amount of glucose consumed during growth was measured with a glucose oxidase test. The AFH medium provided luxuriant growth of all tested strains. The bacterial yield in the fluid medium was 1 times 10 10 ‐1 times 10 11 per ml and overnight cultures on the solid medium resulted in colonies 1–2 mm in diameter.