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A NEW BACTERIOPHAGE TYPING SET FOR PSEUDOMONAS AERUGINOSA
Author(s) -
Bergan Tom
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00148.x
Subject(s) - jaccard index , lytic cycle , phage typing , typing , pseudomonas aeruginosa , bacteriophage , similarity (geometry) , biology , combinatorics , set (abstract data type) , mathematics , genetics , microbiology and biotechnology , computational biology , statistics , computer science , cluster analysis , artificial intelligence , bacteria , virus , escherichia coli , gene , image (mathematics) , programming language
The selection procedure for a new bacteriophage typing set for Pseudomonas aeruginosa is described. The lytic spectra of 113 internationally available phages derived mostly from previous typing sets, had been grouped by numerical allocating techniques applying the unweighted pair group average cluster analysis to the Jaccard‐Sneath similarity matrix and the matrix for the transformed Yule correlation coefficient. This had rendered dendrograms demonstrating the similarity existing between the lytic spectra of the various phages. From each of the clusters arising above a phenon line drawn at a level with approximately 25 clusters, one representative phage was selected. Accordingly, a series of alternative sets were constructed and analysed for their best conformity to the criteria: (a) lysis of the largest possible percentage of bacterial strains, (b) good differentiation, and (c) convenient number of reactions per plate. On this basis a new primary phage typing set for P. aeruginosa of 19 phages and an auxiliary phage typing set of 5 phages were selected.