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The Location of Carbonic Anhydrase in a Strain of Neisseria Flava
Author(s) -
Forkman Anders
Publication year - 1972
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1972.tb00061.x
Subject(s) - spheroplast , carbonic anhydrase , enzyme , strain (injury) , biochemistry , chemistry , bacteria , neisseria , microbiology and biotechnology , lactic dehydrogenase , bacterial cell structure , biology , escherichia coli , genetics , anatomy , gene
The subcellular location of carbonic anhydrase in a strain of Neisseria flava was saught by testing for enzyme activity in whole bacteria, ampicillin‐induced spheroplasts, and a fraction of cells obtained after disruption by freeze‐pressing. The carbonic anhydrase activity of bacterial suspensions disappeared during spheroplast formation, but the activity of a membrane bound enzyme, succinic dehydrogenase, increased. In particulate cell preparations the carbonic anhydrase activity was retained through repeating washing. The results indicate that carbonic anhydrase is located in the cell wall of Neisseria flava.