Premium
INHIBITION OF TRANSFORMATION IN NEISSERIA MENINGITIDIS BY ACRIFLAVIN AND ETHIDIUM BROMIDE
Author(s) -
Jyssum Kaare,
Gundersen Wenche Blix
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb03812.x
Subject(s) - ethidium bromide , transformation (genetics) , neisseria meningitidis , dna , chemistry , transformation efficiency , bromide , rna , microbiology and biotechnology , biochemistry , bacteria , biology , gene , genetics , inorganic chemistry , agrobacterium
Ethidium bromide and acriflavin at low concentration were found to inhibit transformation in Neisseria meningitidis. The transformation efficiency gradually decreased with increasing concentrations of the drugs. In the presence of RNA the effect of the drugs was reduced. The uptake of 3 H‐labelled DNA by the recipient cells was also affected, although not as strongly as the transformation efficiency. This shows that N. meningitidis lacks a step in the DNA uptake that is to these drugs.