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ENZYMOSEROLOGICAL RELATIONSHIPS BETWEEN VIBRIO COMMA AND OTHER GRAM‐NEGATIVE ORGANISMS
Author(s) -
Sandvik Olav,
Dahle Hans Kolbein
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1971.tb02156.x
Subject(s) - vibrio , microbiology and biotechnology , biology , antiserum , casein , enzyme , aeromonas , bacteria , proteolytic enzymes , pseudomonas , pseudomonas aeruginosa , gram , biochemistry , genetics , antibody
Seven tested strains of Vibrio comma and two of Vibrio metschnikovii were all found to produce so‐called Casein Precipitating enzymes (CP‐enzymes). CP‐enzymes produced by the different Vibrio strains, and proteolytic strains of 6 other Gram‐negative bacterial genera have been serologically compared. The technique used is based upon electrophoretic separation of the naturally occurring inhibitors from the induced antiproteinases, combined with the Casein Precipitation Inhibition test, by which the inhibitory activity of the antisera can be analyzed. The analyses indicate that some proteinases of different strains of V. comma are serologically different, while a close enzymoserological relationship exists between others. This relationship may involve only one of two, or more, factors of the enzyme complex. This kind of partial enzymoserological relationship was also found between proteinases from strains of V. comma on the one hand, and Aeromonas liquefaciens and Pseudomonas aeruginosa on the other. The two strains of V. metschnikovii seemed to be enzymoserologic‐ally identical and unrelated to all other organisms tested. The taxonomical significance of the observations has been discussed.