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GROWTH OF HUMAN BRAIN TUMOUR EXPLANTS IN MATRIX CULTURES IN DIFFERENT HUMAN SERA
Author(s) -
Holmström Teddy,
Saksela Eero
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1971.tb01837.x
Subject(s) - explant culture , pathology , matrix (chemical analysis) , in vitro , fibrin , tissue culture , penetration (warfare) , biology , medicine , immunology , chemistry , biochemistry , chromatography , operations research , engineering
The effect of various types of fresh human sera ( Penttinen and Saxén 1957) on the growth and maintenance of 22 clinically benign and 13 clinically malignant human brain tumours was studied in a three‐dimensional matrix culture system using fibrin foam as supporting matrix. The viability and the penetration of the primary explants into the matrix was studied histologically in serial sections. The variation in serum effects on the growth of benign tumours was negligible, whereas the fresh autologous sera and non‐clumping sera supported the growth of malignant tumours significantly better than the aged serum pool and clumping sera. Closer analysis revealed this difference to be due to the significant inhibition of the growth of malignant gliomas under the conditions mentioned. The investigated sera had no effect on histological structure and differentiation of tumour explants in vitro.