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THE METAL (SULPHIDE SILVER METHOD) AND ENZYME CYTOCHEMICAL PATTERN OF HUMAN SEMINAL SPERMATOZOA
Author(s) -
Boström Kerstin
Publication year - 1971
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1971.tb01830.x
Subject(s) - diaphorase , lactate dehydrogenase , succinic dehydrogenase , esterase , acid phosphatase , biochemistry , alkaline phosphatase , dehydrogenase , chemistry , biology , enzyme
Ejaculates from 200 men were examined by the sulphide silver method for the demonstration of metals and by cytochemical methods for the demonstration of lactate and succinic dehydrogenase, NADH‐diaphorase, acid and alkaline phosphatase and nonspecific esterase. By the sulphide silver method, a band‐shaped pattern of silver precipitates (sulphide silver pattern, SSP) appeared in the middle piece of the spermatozoa. 5–9 transverse or oblique bands was regarded as normal. Spermatozoa classified as normally outlined as well as spermatozoa classified as abnormally outlined sometimes showed irregularities in the SSP of the middle piece. The sulphide silver method thus provides a possibility for a further differentiation of spermatozoa. Also by cytochemical methods for the demonstration of lactate and succinic dehydrogenase and NADH‐diaphorase, a band‐shaped pattern appeared in the middle piece, though somewhat less distinct than the SSP. A statistically significant correlation between the percentage of spermatozoa with a regular SSP of the middle piece and the percentage of motile spermatozoa was found.